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cyclin d1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc cyclin d1
    Cyclin D1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 718 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cyclin+d1/Cyclin+D1+XP+Rabbit+mAb/pm42883230-257-39-41
    Average 96 stars, based on 718 article reviews
    cyclin d1 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Incubation:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: .. Membranes were subsequently incubated overnight at 4 °C with specific primary antibodies: β-actin (#4970; 1 : 1000), HPK1 (#46510; 1 : 1000), cyclin D1 (#55506; 1 : 1000), P21 (#2947; 1 : 1000), ERK (#4696; 1 : 1000), phosphorylated ERK (p-ERK, #4370; 1 : 1000), P38 MAPK (#8690; 1 : 1000), and phosphorylated P38 MAPK (p-P38, #9211; 1 : 1000) (all from Cell Signaling Technology, Danvers, Massachusetts, USA). .. Following three 15-min TBST washes, membranes were incubated for 1 h at room temperature with species-matched horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit HS101-01 and goat anti-mouse HS201-01; TransGen Biotech, Beijing, China).

    Article Title: Phytochemical Profiles and Anticancer Activity of the Three Prosopis Species Growing in Qatar Against Lung and Ovarian Cancer Cells.
    Article Snippet: An amount of 20 μg of protein lysates was resolved using 10% SDS-PAGE and transferred to a PVDF membrane (Immobilon PVDF; Biorad, Hercules, CA, USA). .. The PVDF membranes were then blocked with 1X blocking buffer (ref 37520, Thermo Fisher Scientific) and incubated overnight at 4 ◦C with the primary antibodies: anti-rabbit Ki67 (ref 28074-1-AP; Proteintech, Rosemont, IL, USA), AKT (CST #9272s, Cell Signaling Technology), cyclin D1 (CST #55506s; Cell Signaling Technology), anti-mouse BCL2 (CST #15071S; Cell Signaling Technology), anti-rabbit BAX D2E11 (CST #5023; Cell Signaling Technology), anti-mouse P38 MAPK (ref 14064-1-AP, Proteintech), anti-rabbit phospho-p38 MAPK Thr180/Tyr182 (CST #9211, Cell Signaling Technology), anti-rabbit Caspase 3 (CST #9662, Cell Signaling Technology), anti-rabbit Caspase-3 (CST #9662; Cell Signaling Technology) for detection of full-length Caspase-3, anti-rabbit cleaved Caspase-3 (Asp175) (CST #9661; Cell Signaling Technology) for detection of cleaved Caspase-3, and anti-mouse β-actin 8H10D10 (CST #3760; Cell Signaling Technology) antibody was used to confirm equal loading of protein samples. .. The membranes were then washed using 1X TBST and incubated for 1.5 h with either goat anti-rabbit (CST #7074; Cell Signaling Technology) or horse anti-mouse IgG (CST #7076; Cell Signaling Technology) HRP-conjugated secondary antibodies.

    Article Title: FBXL18 activating AKT/CCND1 signaling pathway mediates radioresistance in esophageal squamous cell carcinoma
    Article Snippet: .. Afterwards, PVDF membranes were incubated with primary antibodies against FBXL18 (1:1,000) (H00080028−M01, Abnova, Walnut, CA), AKT (1:1,000) (4691s, CST, MA, USA), p–AKT (1:1,000) (4060, CST), HA (1:5,000) (3724, CST), and Cyclin D1 (1:1,000) (#2922, CST) overnight at 4°C. .. Incubation with mouse antibodies against GAPDH (1:5,000) (sc–32233, Santa Cruz, Calif., USA) was conducted for internal control.

    Cell-Signaling:

    Article Title: Hematopoietic progenitor kinase 1 inhibitor BGB-15025 induces apoptosis in acute myeloid leukemia cells through the cell cycle pathway and mitogen-activated protein kinase/extracellular signal-regulated kinase pathway signaling axis
    Article Snippet: .. Membranes were subsequently incubated overnight at 4 °C with specific primary antibodies: β-actin (#4970; 1 : 1000), HPK1 (#46510; 1 : 1000), cyclin D1 (#55506; 1 : 1000), P21 (#2947; 1 : 1000), ERK (#4696; 1 : 1000), phosphorylated ERK (p-ERK, #4370; 1 : 1000), P38 MAPK (#8690; 1 : 1000), and phosphorylated P38 MAPK (p-P38, #9211; 1 : 1000) (all from Cell Signaling Technology, Danvers, Massachusetts, USA). .. Following three 15-min TBST washes, membranes were incubated for 1 h at room temperature with species-matched horseradish peroxidase-conjugated secondary antibodies (goat anti-rabbit HS101-01 and goat anti-mouse HS201-01; TransGen Biotech, Beijing, China).

    Article Title: SKA2 promotes gastric cancer progression by regulating glutathione metabolism
    Article Snippet: Flag-Tag , Sigma-Aldrich , Cat#F1804; RRID: AB_262044. .. Cyclin D1 , Cell Signaling Technology , Cat#2922; RRID: AB_2228523. .. Cyclin A2 , Cell Signaling Technology , Cat#4656; RRID: AB_2071958.

    Article Title: Phytochemical Profiles and Anticancer Activity of the Three Prosopis Species Growing in Qatar Against Lung and Ovarian Cancer Cells.
    Article Snippet: An amount of 20 μg of protein lysates was resolved using 10% SDS-PAGE and transferred to a PVDF membrane (Immobilon PVDF; Biorad, Hercules, CA, USA). .. The PVDF membranes were then blocked with 1X blocking buffer (ref 37520, Thermo Fisher Scientific) and incubated overnight at 4 ◦C with the primary antibodies: anti-rabbit Ki67 (ref 28074-1-AP; Proteintech, Rosemont, IL, USA), AKT (CST #9272s, Cell Signaling Technology), cyclin D1 (CST #55506s; Cell Signaling Technology), anti-mouse BCL2 (CST #15071S; Cell Signaling Technology), anti-rabbit BAX D2E11 (CST #5023; Cell Signaling Technology), anti-mouse P38 MAPK (ref 14064-1-AP, Proteintech), anti-rabbit phospho-p38 MAPK Thr180/Tyr182 (CST #9211, Cell Signaling Technology), anti-rabbit Caspase 3 (CST #9662, Cell Signaling Technology), anti-rabbit Caspase-3 (CST #9662; Cell Signaling Technology) for detection of full-length Caspase-3, anti-rabbit cleaved Caspase-3 (Asp175) (CST #9661; Cell Signaling Technology) for detection of cleaved Caspase-3, and anti-mouse β-actin 8H10D10 (CST #3760; Cell Signaling Technology) antibody was used to confirm equal loading of protein samples. .. The membranes were then washed using 1X TBST and incubated for 1.5 h with either goat anti-rabbit (CST #7074; Cell Signaling Technology) or horse anti-mouse IgG (CST #7076; Cell Signaling Technology) HRP-conjugated secondary antibodies.

    Electrophoresis:

    Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
    Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

    Membrane:

    Article Title: Compositions and methods for increasing cancer cell sensitivity to alternating electric fields
    Article Snippet: Samples were run on SDS-polyacrylamide gel electrophoresis (Bolt 8% Bis-Tris base gel NW00080BOX, Thermo-Fischer). .. After electrophoresis, proteins were transferred to 0.2 μm polyvinylidene difluoride membrane (Immuno-Blot PVDF #162-0177, Bio-Rad) and probed with the appropriate primary antibody: GAPDH (SC-32233, Santa Cruz), Vinculin (ab140007, Abcam), Cyclin D1 (2922, Cell Signaling), Rb (9309, Cell Signaling), pRb S807/811 (8516, Cell Signaling), pAkt 5473, (4051, Cell signaling), Akt (2920, Cell signaling)), FAK (3283S, Cell signaling), Phospho-FAK (Tyr397) (3283S, Cell signaling, Src (2109S, cell signaling) and Phospho-Src (Tyr416) (2101S, Cell signaling). followed by horseradish peroxidase-conjugated secondary antibody (goat anti rabbit 7074, Cell Signaling and goat anti mouse 7076, Cell Signaling) and a chemiluminescent substrate (WBLUF0100, Signa-Aldrich). ..

    Blocking Assay:

    Article Title: Phytochemical Profiles and Anticancer Activity of the Three Prosopis Species Growing in Qatar Against Lung and Ovarian Cancer Cells.
    Article Snippet: An amount of 20 μg of protein lysates was resolved using 10% SDS-PAGE and transferred to a PVDF membrane (Immobilon PVDF; Biorad, Hercules, CA, USA). .. The PVDF membranes were then blocked with 1X blocking buffer (ref 37520, Thermo Fisher Scientific) and incubated overnight at 4 ◦C with the primary antibodies: anti-rabbit Ki67 (ref 28074-1-AP; Proteintech, Rosemont, IL, USA), AKT (CST #9272s, Cell Signaling Technology), cyclin D1 (CST #55506s; Cell Signaling Technology), anti-mouse BCL2 (CST #15071S; Cell Signaling Technology), anti-rabbit BAX D2E11 (CST #5023; Cell Signaling Technology), anti-mouse P38 MAPK (ref 14064-1-AP, Proteintech), anti-rabbit phospho-p38 MAPK Thr180/Tyr182 (CST #9211, Cell Signaling Technology), anti-rabbit Caspase 3 (CST #9662, Cell Signaling Technology), anti-rabbit Caspase-3 (CST #9662; Cell Signaling Technology) for detection of full-length Caspase-3, anti-rabbit cleaved Caspase-3 (Asp175) (CST #9661; Cell Signaling Technology) for detection of cleaved Caspase-3, and anti-mouse β-actin 8H10D10 (CST #3760; Cell Signaling Technology) antibody was used to confirm equal loading of protein samples. .. The membranes were then washed using 1X TBST and incubated for 1.5 h with either goat anti-rabbit (CST #7074; Cell Signaling Technology) or horse anti-mouse IgG (CST #7076; Cell Signaling Technology) HRP-conjugated secondary antibodies.

    Hemagglutination Assay:

    Article Title: FBXL18 activating AKT/CCND1 signaling pathway mediates radioresistance in esophageal squamous cell carcinoma
    Article Snippet: .. Afterwards, PVDF membranes were incubated with primary antibodies against FBXL18 (1:1,000) (H00080028−M01, Abnova, Walnut, CA), AKT (1:1,000) (4691s, CST, MA, USA), p–AKT (1:1,000) (4060, CST), HA (1:5,000) (3724, CST), and Cyclin D1 (1:1,000) (#2922, CST) overnight at 4°C. .. Incubation with mouse antibodies against GAPDH (1:5,000) (sc–32233, Santa Cruz, Calif., USA) was conducted for internal control.



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